体外抑制Piwi基因对鸡原始生殖细胞相关基因表达的影响

doi:10.11843/j.issn.0366-6964.2014.06.003

Abstract

Abstract:

This study was designed to interfere Piwi gene in chicken primordial germ cells (PGCs) and explore its interaction with CVH，Dazl，CDH, Sox2, Nanog and PouV genes.PGCs was isolated from 19 stage chicken embryo，and after identification of PGCs，we analyzed expression of CVH，Dazl，CDH，Nanog，Sox2 and PouV under the condition of Piwi interference in PGCs by using real time quantitative PCR.The results showed that expression of CVH，Dazl and Nanog genes increased significantly(P<0.05)，while the expression of PouV and Sox2 genes decreased significantly followed with Piwi interference in PGCs (P<0.05).Collectively，we constructed a Piwi gene interference model in vitro and found,and which influenced expression of CVH，Dazl，CDH, Sox2，Nanog and PouV，which provided new basis for studying Piwi gene function in reproductive biology.

CLC Number:

S831.2

LI Jian-chao，ZHANG Ying，DAI Ai-qin，WANG Hong-zhi，ZHAI Fei，HUA Deng-ke，XIA Ming-xiu， CHANG Guo-bin，CHEN Guo-hong. In Vitro Inhibition of Piwi Gene Expression and the Influence on Related Genes in Primordial Germ Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(6): 871-878.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2014.06.003

https://www.xmsyxb.com/EN/Y2014/V45/I6/871

References

［1］LIN H，SPRADLING A C.A novel group of pumilio mutations affects the asymmetric division of germline stem cells in the Drosophila ovary ［J］.Development，1997，124:2463-2476.
［2］COX D N，CHAO A，LIN H.Piwi encodes a nucleoplasmic factor whose activity modulates the number and division rate of germline stem cells ［J］.Development，2000，127:503-514.
［3］KURAMOCHI-MIYAGAWA S，KIMURA T，LJIRI T W，et al.Mili，a mammalian member of Piwi family gene，is essential for spermatogenesis ［J］.Development，2004，131:839-849.
［4］DENG W，LIN H.miwi，a murine homolog of Piwi，encodes a cytoplasmic protein essential for spermatogenesis ［J］.Developmental Cell，2002，2(6)：819-830.
［5］QIAO D，ZEEMAN A M，DENG W，et al.Molecular characterization of hiwi，a human member of the Piwi gene family whose overexpression is correlated to seminomas ［J］.Oncogene，2002，21(25):3988-3999.
［6］ARAVIN A，GAIDATZIS D，PFEFFER S，et al.A novel class of small RNAs bind to MILI protein in mouse testes ［J］.Nature，2006，442(7099)：203-207.
［7］GORARD A，SACHIDANANDAM R，HANNO G J，et al.A germline-specific class of small RNAs binds mammalian Piwi proteins ［J］.Nature，2006，442(7099)：199-202.
［8］GRIVNA S T，BEYRET E，WANG Z，et al.A novel class of small RNAs in mouse spermatogenic cells ［J］.Genes Dev，2006，20(13)：1709-1714.
［9］LAU N C，STEO A G，KIM J，et al.Characterization of the piRNA complex from rat testes ［J］.Science，2006，313(5785)：363-367.
［10］WZTANABE T，TAKEDA A，TSUKIYAMA T，et al.Identification and characterization of two novel classes of small RNAs in the mouse germline：retrotransposon-derived siRNAs in oocytes and germline small RNAs in testes ［J］.Genes Dev，2006，20(13)：1732-1743.
［11］HAMBURGER V，HAMILTON H L.A series of normal stages in the development of the chick embryo ［J］.J Morphol，1951，88(1)：49-92.
［12］朱国坡，周晓丽，郭延锋，等.鸡胚成纤维细胞原代培养及应用［J］.动物医学进展，2010，31（3）：112-114.
［13］TANG X Y，ZHANG C Q，ZENG W D，et al.Proliferating effects of the flavonoids daidzein and quercetin on cultured chicken primordial germ cells through antioxidant action ［J］.Cell Biol Int，2006，30：445-451.
［14］PARK T S，HAN J Y.Derivation and characterization of pluripotent embryonic germ cells in chicken ［J］.Mol Reprod Dev，2000，56:475-482.
［15］NAITO M，TAJIMA A，TAJIMA T，et al.Preservation of chick primordial germ cells in liquid nitrogen and subsequent production of viable offspring ［J］.J Reprod Fert，1994，102(2)：321-325.
［16］TSUNEKAWA N，NAITO M，SAKAI Y，et al.Isolation of chicken vasa homolog gene and tracing the origin of primordial germ cells ［J］.Development，2000，127（12）:2741-2750.
［17］MURAMATSU T，MURAMATSU H.Carbohydrate antigens expressed on stem cell and early embryonic cells ［J］.Glycoconj J，2004，21(1-2):41-45.
［18］TRUDI S，ERIC W.Maternal-effect mutations altering the anterior-posterior pattern of the Drosophila embryo ［J］.Roux’s Arch Dev Biol，1986，195:302-317.
［19］THOMSON T，LIN H.The biogenesis and function PIWI proteins and piRNAs：progress and prospect ［J］.Annu Rev Cell Dev Biol，2009，25：355-376.
［20］LIN Y M，CHEN C W，SUN H S，et al.Expression patterns and transcript concentrations of the autosomal DAZL gene in testes of azoospermic men ［J］.Mol Hum Reprod，2001，7(11)：1015-1022.
［21］YEN P H，CHAI N N，SALIDO E C，et al.The human autosomal gene DAZLA：testis specificity and a candidate for male infertility ［J］.Hum Mol Genet，1996，5(12)：2013-2017.
［22］WEIDINGER G，STEBLER J，SLANCHEV K，et al.Dead end，a novel vertebrate germ plasm component，is required for zebrafish primordial germ cell migration and survival ［J］.Curr Biol，2003，13(16)：1429-1434.
［23］YOUNGREN K K，COVENEY D，PENG X，et al.The Ter mutation in the dead end gene causes germ cell loss and testicular germ cell tumours ［J］.Nature，2005，435(7040)：360-364.
［24］ARAMAKI S，SATO F，KATO T，et al.Molecular cloning and expression of dead end homologue in chicken primordial germ cells ［J］.Cell Tissue Res，2007，330(1)：45-52.
［25］RODDA D J，CHEW J L，LIM L H，et al.Transcriptional regulation of nanog by OCT4 and SOX2［J］.J Biol Chem，2005，280(26)：24731-24737.
［26］LAVIAL F，ACLOQUE H，BERTOCCHINI F，et al.The Oct4 homologue PouV and Nanog regulate pluripotency in chicken embryonic stem cells ［J］.Development，2007，134(19)：3549-3563.
［27］MASUI S，NAKATAKE Y，TOUOOKA Y，et al.Pluripotency governed by Sox2 via regulation of Oct3/4 expression in mouse embryonic stem cells ［J］. Nat Cell Biol，2007，9(6)：625-635.
［28］WANG Z，ORON E，NELSON B，et al.Distinct lineage specification roles for NANOG，OCT4，and SOX2 in human embryonic stem cells［J］.Cell Stem Cell,2012,10(4):440-454.

[1]	TANG Xinxin, ZHENG Jumei, LUO Na, YING Fan, ZHU Dan, LI Sen, LIU Dawei, AN Bingxing, WEN Jie, ZHAO Guiping, LI Hegang. Genetic Mechanism of Broiler Leg Disease Based on Genome-Wide Association Analysis [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 99-109.
[2]	XU Xiaojing, DONG Ruiling, WEI Limin, ZHAO Shaomeng, ZHAO Guiping, ZHANG Minhong, FENG Jinghai. Study on Growth Model of Wenchang Chickens [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 4952-4961.
[3]	QI Lina, LU Xuelin, YANG Kaixuan, ZHOU Jin, LI Xianyu, LIU Ke, ZHANG Wengang, GU Xin, ZHANG Weijian. Analysis of Genetic Diversity and Genetic Structure of New Pudong Chicken Based on SNP Chips [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 4962-4971.
[4]	WANG Yanxing, ZHANG Yushi, JI Haigang, LIU Yang, NIU Yufang, HAN Ruili, LIU Xiaojun, TIAN Yadong, KANG Xiangtao, LI Zhuanjian. Establishment and Analysis of the Chicken Skeletal Satellite Cell Line [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 4972-4981.
[5]	FAN Chenyu, SHAN Yanju, ZHANG Ming, JI Gaige, JU Xiaojun, TU Yunjie, HE Xi, SHU Jingting, LIU Yifan, ZHANG Haihan. Genome-wide Association Study of Body Weight and Meat Quality Traits in Lihua Mahuang Chickens [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 4982-4992.
[6]	CHEN Cheng, QIAO Xibo, SUN Yi, KANG Li, JIANG Yunliang. Study on the Polymorphism and Genetic Effect of the -868 Locus of FSHR Gene on Egg Laying Traits in Langya Chickens [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4560-4568.
[7]	JIN Sihua, JIA Fumin, HE Peili, JIA Yuqing, SHUI Fei, LIU Xuling, LIU Xing, WANG Xin, XU Man, GENG Zhaoyu. Effect of IHH Gene Overexpression on Proliferation and Differentiation of Chicken Primary Chondrocytes [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4569-4576.
[8]	CHEN Xuejiao, LIU Huijie, ZANG Lei, FENG Jing, PENG Da, ZHANG Hao. Transcriptome Data from Chicken Embryo Heart Tissue Identified Key Genes for Altitude Hypoxia Adaptation in Xueyu White Chickens [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4154-4163.
[9]	ZHANG Yinliang, YUE Qiaoxian, HUANG Chenxuan, CHEN Hui, WANG Dehe, ZHOU Rongyan. Construction of Tibia Transcript Profile of Laying Hens at the Early and Late Laying Stages and Analysis of Genes Related to Bone Metabolism [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4164-4173.
[10]	ZHENG Gang, LIAN Ling. Research Progress of Key Regulatory Gene DMRT1 in Chicken Sex Determination and Differentiation [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3152-3163.
[11]	BAI Lu, WANG Mengjie, MA Xiaochun, HE Zhengxiao, TAN Xiaodong, LIU Jie, ZHAO Guiping, WEN Jie, LIU Ranran. A Method for Quickly Mining the Characteristic SNP Markers Set of Chicken [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3252-3261.
[12]	CHEN Chun, KANG Zhaofeng, WEI Yue, LI Guanhong, WU Yanping, XIE Jinfang. Correlation of Wnt3a Gene Polymorphism with Skin Follicle Traits in Chongren Partridge Chicken [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2810-2823.
[13]	LIU Hang, WANG Huanhuan, GE Ying, ZHANG Lei, ZHANG Weiwu, WEI Yinghui, LI Qinghai, FAN Jinghui, ZHANG Xuedong. Screening of Candidate Genes of Skin Color of Black-Bone Chicken Based on Transcriptome and Proteome [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2320-2329.
[14]	WANG Hui, XU Mengyuan, LIU Lingkang, ZHENG Xibang, LI Gonghe, WU Wende. PhiC31 Integrase Triggers Site-specific Cassette Exchange in Chicken Fibroblast Cells through Electroporation [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2330-2342.
[15]	WANG Zheng, GUO Wenjie, CHENG Jin, YUAN Yitong, LUO Rong, XUE Yi, ZHANG Lihuan, ZHU Zhiwei, LI Huifeng. Association Study of Polymorphism in the Regulatory Region of Nutrient Transport-related Genes and Feed Conversion Ratio in Yellow-feather Broiler [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2343-2352.

In Vitro Inhibition of Piwi Gene Expression and the Influence on Related Genes in Primordial Germ Cells

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments